Compact Dry LIS is a ready to use, chromogenic plate for detection of Listeria species.
Viable count in water or liquid foodstuff
Drop 1 ml of specimen (dilute if necessary) on the middle of the Compact Dry plate.
Viable count in solid foodstuff
Add buffer solution to the sample and homogenize by stomacher®. Drop 1 ml of specimen (dilute if necessary) on the middle of the dry sheet of the Compact Dry plate.
Viable count in swab test specimen
Use the swab to wipe the surface, put into the device with wiping solution. Drop 1 ml of wiping solution (dilute if necessary) on the middle of the Compact Dry plate. It is recommended to use “Swab for Compact Dry” offered by HyServe Id-No. 1 002 952/3 (40/240 pieces)
1. Open the cap and drop 1 ml of specimen on the middle of the Compact Dry plate.
2. Specimen diffuses automatically and evenly into the sheet and transforms the dried sheet into a gel within seconds.
3. Put the cap again on the plate and write the information needed on the memorandum section.
4. Turn over the capped plate and put in the incubator.5. After incubation count the number of colored colonies underneath the plate. White paper placed under the plate helps to count the colonies.
Incubation time: 24 ± 2 hours
Incubation temperature: 37 ± 1 °C
Interpretation of the results: Listeria species forms light blue/blue colonies of 1~2 mm in diameter by chromgens contained in a medium.
Storage: Room temperature (+ 1 to +30 °C).
Shelf Life: 12 months after manufacturing.
It is known that Listeria ivanovii tends to grow slowely and Listeria seeligeri tends to be inhibited in this plate.
A combination of selective agents in the medium inhibit the growth of other bacteria.
High concentrations on plates will cause the entire growth area to become red/pink. In this case dilute the sample.
After use please follow the current disposal regulations.
The growth area is 20 cm². The back of the plate has a grid carved of 1 cm x 1 cm to make the colony counting easier. In case of any difficulties to count colonies due to large number of colonies grown, total viable count can be obtained by multiplying 20 by an average number of colonies per grid counted from several grids.
Compact Dry plates are produced at an ISO 9001 certified site.