The tethaPatch™ six separate chambers of the tethaPlate can be addressed individually for convenient replicate experiments, or separate experiments can be performed in each chamber.
Features & Functions
By assembling a single type of ion channel protein in a large patch (2.1 mm2 area) of artificial tethered membrane somewhere between 1 and 10 million channels can be studied in parallel.
The measured ion current is the sum total from all the ion channels in the patch so that signals in the microampere ranges are obtained with concomitantly low noise levels.
The ion channels spontaneously house themselves in the tethered membrane which is already bonded to the working electrode. There is no 'hit-or-miss' approach with a micropipette electrode.
TethaPatch™ techniques, typically provide results in minutes that would otherwise take hours with conventional techniques.
Tethered membranes are very robust, voltage pulses can be applied that would otherwise 'blow' the seal of a traditional patch clamp preparation. Thus voltage-gated, and mechanosensitive ion channels can now be studied over a wide range of pulse amplitudes (potentials between -500 to +800 mV can be applied) than previously achievable (typically less than ±300 mV), leading to new insights into the mechanism of ion channel behaviour.
The ER466 Integrated Potentiostat is suitable for use with the tethaPatch for pulse generation and data acquisition of the current signal.
eDAQ | Laboratory instruments & Data acquisition systems
eDAQ manufactures laboratory instruments and data acquisition systems for:
Electrochemistry, Chromatography, Flow analysis, Contactless conductivity, Sensor recording, Electrochemical impedance, Teaching ...