IncuCyte® NucLight Rapid Red Reagent

The IncuCyte NucLight Rapid Red Reagent for nuclear labeling is a cell permeable DNA stain that specifically stains nuclei in cells and is ideally suited to the mix-and-read, real-time quantification using the IncuCyte® Live-Cell Analysis System. Addition of the IncuCyte NucLight Rapid Red Reagent to normal healthy cells is non-perturbing to cell growth and morphology and provides homogenous staining of nuclei, enabling the real-time, fluorescent quantification of cell proliferation. When added to tissue culture medium, the inert stain crosses the cell membrane and has excellent specificity for DNA without the need for a wash step. With the IncuCyte® integrated analysis software, fluorescent objects can be quantified and background fluorescence minimized. This reagent can be multiplexed with IncuCyte® Annexin V, Caspase-3/7 or Cytotox reagents for simultaneous readouts of apoptosis or cytotoxicity alongside cell proliferation.

Key advantages:

• Simple to use, mix-and-read reagent
• Rapid, high efficiency cell labeling
• Non-perturbing to cells at optimized dye concentrations
• Ideal for homogenous labeling of multiple cell types