HPLC Pump in Perth
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HPLC Pump Suppliers in Perth
Thermo Fisher Scientific Australia Pty Ltd is an HPLC Pump supplier to All
Thermo Fisher Scientific is a leading provider of instruments, supplies, and services for the environmental, industrial, municipal water, marine environment and global scientific markets. We offer the most up-to-date technologies from the world's leading manufacturers and supply an extensive range of testing, measurement, monitoring, control devices and associated consumables ...Thermo Scientific Vanquish™ Isocratic Pump C
UltiMate™ HPG-3200BX Biocompatible Binary Semi Preparative Pump
Thermo Scientific Vanquish™ Charger Module
Scientronic Instrument Services is an HPLC Pump supplier to Perth
PERTHS LEADING DISTRIBUTOR OF ANALYTICAL AND SCIENTIFIC SUPPLIES Scientronic Services focuses on optimizing your Millipore, Waters and Dionex products with superior service, support, training, upgrades, and quality parts. Whether your company is a single-location lab or a large organization, Scientronic Instrument Services provides you with the expertise and responsiveness ...
- Reverse-phase HPLC: This is the most common type of HPLC used in Australia. It is used to separate non-polar or weakly polar compounds.
- Normal-phase HPLC: This type of HPLC is used to separate polar or ionic compounds.
- Ion-exchange HPLC: This type of HPLC is used to separate charged molecules based on their charge.
- Size-exclusion HPLC: This type of HPLC is used to separate molecules based on their size.
- Affinity HPLC: This type of HPLC is used to separate molecules based on their specific binding properties.
- Chiral HPLC: This type of HPLC is used to separate enantiomers (mirror-image isomers) of a molecule.
- Pump: It is used to deliver the mobile phase (solvent) under high pressure to the column to maintain a constant flow rate.
- Injector: It is used to introduce the sample into the mobile phase stream and onto the column.
- Column: It is the heart of the HPLC system, where the separation of the components in the sample occurs.
- Detector: It is used to detect and measure the separated components as they elute from the column.
- Data acquisition system: It is used to record and analyze the data generated by the detector.
- Mobile phase: It is a solvent or a mixture of solvents that is used to elute the sample components from the column.
- Stationary phase: It is a material that is packed into the column and used to separate the components in the sample based on their physical and chemical properties.
- Normal phase HPLC uses a polar stationary phase (such as silica gel) and a nonpolar mobile phase (such as hexane or heptane). In this technique, the polar molecules in the sample interact with the polar stationary phase, while the nonpolar molecules interact with the nonpolar mobile phase. This technique is useful for separating polar compounds such as amino acids, peptides, and carbohydrates.
- Reverse phase HPLC, on the other hand, uses a nonpolar stationary phase (such as C18) and a polar mobile phase (such as water or methanol). In this technique, the nonpolar molecules in the sample interact with the nonpolar stationary phase, while the polar molecules interact with the polar mobile phase. This technique is useful for separating nonpolar compounds such as lipids, steroids, and hydrophobic peptides.
- UV-Visible Detector: This is the most commonly used detector in HPLC. It measures the absorption of light by the sample at a specific wavelength. It is suitable for analyzing compounds that absorb UV or visible light.
- Fluorescence Detector: This detector is used to detect fluorescent compounds. It measures the emission of light by the sample after being excited by a light source.
- Refractive Index Detector: This detector is particularly useful for analyzing compounds that do not absorb UV or visible light, such as sugars and polymers. It measures the difference in the refractive index of the sample and the mobile phase.
- Electrochemical Detector: This detector measures the electrical potential generated by the oxidation or reduction of a compound. It is particularly useful for analyzing electroactive compounds such as neurotransmitters and drugs.
- Mass Spectrometry Detector: This detector is used to identify and quantify compounds based on their mass-to-charge ratio. It is particularly useful for analyzing complex mixtures and for identifying unknown compounds.
- Check the solvent levels: Ensure that the solvents used in the HPLC system are at the required levels. If the levels are low, add more solvents to avoid any errors.
- Check the column: Inspect the HPLC column for any damage or clogging. If there is any damage or clogging, it needs to be cleaned or replaced.
- Check the injection system: Make sure that the injection system is clean and free from any blockages. Clean the injection system regularly to avoid any errors.
- Check the detector: Inspect the detector for any damage or contamination. Clean the detector regularly to avoid any errors.
- Check the pumps: Check the pumps for any damage or leaks. If there is any damage or leaks, it needs to be repaired or replaced.
- Check the software: Ensure that the software used in the HPLC system is up-to-date and working correctly.
- Calibration: Calibrate the HPLC system regularly to maintain its accuracy and reliability.
- System suitability test: Perform a system suitability test before using the HPLC system to ensure that it is working correctly.
HPLC Pump Costs and Supplier Insights
If you are looking to buy a HPLC Pump for sale, suppliers on IndustrySearch include Thermo Fisher Scientific Australia Pty Ltd
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