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HPLC Pump in Melbourne
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Looking to buy the best HPLC Pump for sale in Melbourne? Get Quotes sends your requirements to expert suppliers in Australia so you get personalised quotes to compare HPLC Pump prices, specifications, features and terms then choose the one that’s right for you. Also compare servicing, consumables and reviews, so you can buy with confidence.
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HPLC Pump Suppliers in Melbourne
Updated Sep 18, 2026
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Thermo Fisher Scientific Australia Pty Ltd |
Thermo Fisher Scientific Australia Pty Ltd is an HPLC Pump supplier to All
Thermo Fisher Scientific is a leading provider of instruments, supplies, and services for the environmental, industrial, municipal water, marine environment and global scientific markets. We offer the most up-to-date technologies from the world's leading manufacturers and supply an extensive range of testing, measurement, monitoring, control devices and associated consumables ...
Thermo Scientific Vanquish™ Isocratic Pump C
The Isocratic Vanquish Pump C delivers highly precise and reliable isocratic liquid chromatography for routine laboratory applications.
UltiMate™ HPG-3200BX Biocompatible Binary Semi Preparative Pump
UltiMate™ HPG-3200BX Biocompatible Binary Semi Preparative Pump
Thermo Scientific Vanquish™ Charger Module
The Vanquish Charger Module is a fully integrated, robotic sample management unit designed for use with the Vanquish UHPLC system, offering ...
Allabin Pty Ltd | Chromatography System
Allabin Pty Ltd is an HPLC Pump supplier to Melbourne
Allabin is a supplier of quality laboratory equipment with relevant consumables and supplies based in Victoria Australia. Our company also focuses on manufacturing, customizing and developing instruments and related applications for uses in analytical chemistry and chemical process control. Allabin's primary target is to provide and manufacture instruments which can meet customer ...
HPLC Pump
HPLC Pump
What is HPLC and what are its applications?
HPLC is a widely used analytical technique that separates, identifies, and quantifies components in a mixture. It employs a liquid mobile phase, a stationary phase, and a high-pressure pump to push the sample through the column. The stationary phase can be made up of a variety of materials, including silica, alumina, or a polymer. Detection is usually accomplished by UV-Vis spectroscopy, fluorescence, or mass spectrometry. HPLC is used in many areas of research, including pharmaceuticals, food and beverages, environmental studies, forensics, and medical diagnostics. It is an essential tool for identifying and quantifying small molecules and biomolecules, such as proteins and peptides, in complex mixtures.
What are the different types of HPLC systems available in Australia?
There are several types of HPLC systems available in Australia, including:
- Reverse-phase HPLC: This is the most common type of HPLC used in Australia. It is used to separate non-polar or weakly polar compounds.
- Normal-phase HPLC: This type of HPLC is used to separate polar or ionic compounds.
- Ion-exchange HPLC: This type of HPLC is used to separate charged molecules based on their charge.
- Size-exclusion HPLC: This type of HPLC is used to separate molecules based on their size.
- Affinity HPLC: This type of HPLC is used to separate molecules based on their specific binding properties.
- Chiral HPLC: This type of HPLC is used to separate enantiomers (mirror-image isomers) of a molecule.
What are the key components of an HPLC system and what do they do?
High Performance Liquid Chromatography (HPLC) is a powerful analytical technique used in the separation, identification, and quantification of various compounds. The key components of an HPLC system include:
- Pump: It is used to deliver the mobile phase (solvent) under high pressure to the column to maintain a constant flow rate.
- Injector: It is used to introduce the sample into the mobile phase stream and onto the column.
- Column: It is the heart of the HPLC system, where the separation of the components in the sample occurs.
- Detector: It is used to detect and measure the separated components as they elute from the column.
- Data acquisition system: It is used to record and analyze the data generated by the detector.
- Mobile phase: It is a solvent or a mixture of solvents that is used to elute the sample components from the column.
- Stationary phase: It is a material that is packed into the column and used to separate the components in the sample based on their physical and chemical properties.
What types of columns are available for HPLC and how do I choose the right one?
There are several types of columns available for HPLC, including reversed-phase, normal-phase, ion-exchange, size-exclusion, and affinity. The choice of column depends on the nature of the sample, the analytes of interest, the desired separation mechanism, and the type of detector being used. For example, reversed-phase columns are commonly used for hydrophobic analytes, while normal-phase columns are suitable for polar analytes. Ion-exchange columns are used for charged analytes, size-exclusion columns are used for separation based on molecular size, and affinity columns are used for binding interactions. The selection of the appropriate column is crucial for achieving high-quality separation and accurate quantification of analytes.
What is the difference between normal phase and reverse phase HPLC?
Normal phase HPLC and reverse phase HPLC are two types of chromatography techniques used in HPLC. The main difference between them is the nature of the stationary phase used in these two techniques.
- Normal phase HPLC uses a polar stationary phase (such as silica gel) and a nonpolar mobile phase (such as hexane or heptane). In this technique, the polar molecules in the sample interact with the polar stationary phase, while the nonpolar molecules interact with the nonpolar mobile phase. This technique is useful for separating polar compounds such as amino acids, peptides, and carbohydrates.
- Reverse phase HPLC, on the other hand, uses a nonpolar stationary phase (such as C18) and a polar mobile phase (such as water or methanol). In this technique, the nonpolar molecules in the sample interact with the nonpolar stationary phase, while the polar molecules interact with the polar mobile phase. This technique is useful for separating nonpolar compounds such as lipids, steroids, and hydrophobic peptides.
What are the different types of detectors used in HPLC and which one should I choose?
High Performance Liquid Chromatography (HPLC) is a technique used to separate, identify, and quantify components in a mixture of chemicals. There are various detectors used in HPLC, each with its own advantages and limitations. The choice of detector depends on the specific requirements of the analysis, such as the type of sample and the desired sensitivity and selectivity.
- UV-Visible Detector: This is the most commonly used detector in HPLC. It measures the absorption of light by the sample at a specific wavelength. It is suitable for analyzing compounds that absorb UV or visible light.
- Fluorescence Detector: This detector is used to detect fluorescent compounds. It measures the emission of light by the sample after being excited by a light source.
- Refractive Index Detector: This detector is particularly useful for analyzing compounds that do not absorb UV or visible light, such as sugars and polymers. It measures the difference in the refractive index of the sample and the mobile phase.
- Electrochemical Detector: This detector measures the electrical potential generated by the oxidation or reduction of a compound. It is particularly useful for analyzing electroactive compounds such as neurotransmitters and drugs.
- Mass Spectrometry Detector: This detector is used to identify and quantify compounds based on their mass-to-charge ratio. It is particularly useful for analyzing complex mixtures and for identifying unknown compounds.
What is the cost of an HPLC system in Australia?
A basic HPLC system can range from $20,000 to $50,000 AUD, while advanced models with multiple detectors and automation options can cost upwards of $100,000 AUD. Additionally, ongoing maintenance, consumables, and specialist training costs should be considered when budgeting for an HPLC system. It's recommended to consult with vendors and compare quotes from multiple suppliers to find the best deal.
What are the maintenance requirements for an HPLC system?
Maintaining an HPLC system is essential to get accurate and reliable results. The following are the maintenance requirements for an HPLC system:
- Check the solvent levels: Ensure that the solvents used in the HPLC system are at the required levels. If the levels are low, add more solvents to avoid any errors.
- Check the column: Inspect the HPLC column for any damage or clogging. If there is any damage or clogging, it needs to be cleaned or replaced.
- Check the injection system: Make sure that the injection system is clean and free from any blockages. Clean the injection system regularly to avoid any errors.
- Check the detector: Inspect the detector for any damage or contamination. Clean the detector regularly to avoid any errors.
- Check the pumps: Check the pumps for any damage or leaks. If there is any damage or leaks, it needs to be repaired or replaced.
- Check the software: Ensure that the software used in the HPLC system is up-to-date and working correctly.
- Calibration: Calibrate the HPLC system regularly to maintain its accuracy and reliability.
- System suitability test: Perform a system suitability test before using the HPLC system to ensure that it is working correctly.
What is the lead time for delivery and installation of an HPLC system?
It depends on several factors such as the availability of the system, the location of the buyer, and the complexity of the installation process. A buyer can contact the manufacturer or supplier of the HPLC system to get an estimated lead time for delivery and installation.
Can you provide training and support for the use of the HPLC system?
Additionally, the manufacturer of the HPLC system may offer training and support services.
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HPLC Pump Costs and Supplier Insights
The listed price of a HPLC Pump for sale ranges from $5,000 to $15,000, averaging $10,000.
If you are looking to buy a HPLC Pump for sale, suppliers on IndustrySearch include Thermo Fisher Scientific Australia Pty Ltd, Allabin Pty Ltd
Use Get Quotes to receive personalised prices and proposals from trusted suppliers with top reviews and excellent service.
Why choose a supplier in Melbourne for HPLC-High Performance Liquid Chromatography Quotes?
If you are in Melbourne and considering purchasing a HPLC-High Performance Liquid Chromatography, it's worth considering Melbourne-based suppliers. Doing so offers several benefits that are particularly relevant to businesses in the area. Here are the top reasons why opting for a local Melbourne supplier can be the best decision for your HPLC-High Performance Liquid Chromatography buying needs.
Immediate Assistance in MelbourneEnjoy immediate assistance and troubleshooting in Melbourne. Fast resolution keeps your operations running smoothly.
Ensuring HPLC-High Performance Liquid Chromatography Quality with Melbourne SuppliersEnsure your HPLC-High Performance Liquid Chromatography meets the highest quality standards with the expert guidance of Melbourne suppliers. Excellence in every detail.
Access to HPLC-High Performance Liquid Chromatography Training Experts in MelbourneGain direct access to HPLC-High Performance Liquid Chromatography training experts. Local suppliers can provide tailored instruction that fits your specific needs and challenges.
Melbourne Providers Leading Innovation AdoptionChoose Melbourne HPLC-High Performance Liquid Chromatography providers leading in innovation adoption. They help you implement the latest industry trends swiftly, enhancing your operational capacity.
Supply Chain Stability with Melbourne SuppliersEnhance your supply chain stability by choosing HPLC-High Performance Liquid Chromatography suppliers in Melbourne. Local sourcing reduces risks associated with global disruptions.
Immediate HPLC-High Performance Liquid Chromatography Availability in MelbourneBenefit from immediate HPLC-High Performance Liquid Chromatography availability when you choose suppliers in Melbourne. Quick access to equipment prevents project delays.
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